Tattoo imaging
FP-based biosensors, and especially those based on Fluorescent Resonance Energy Transfer (FRET), can report on cellular processes such as apoptosis or cell division, the levels of second messengers such as Ca 2+, PtdIns(4,5) P 2 and cAMP, or the activation status of proteins such as RhoA.
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The introduction of fluorescent proteins (FP) and the ability to visualize molecular events using fluorescence microscopy have been of substantial value for understanding intracellular signaling events.
Tattoo imaging skin#
This "instant skin transgenic" model can be used to follow the in vivo behavior of individual cells expressing either FRET- or location-based probes for several days after tattooing and provides a rapid and inexpensive method for intravital imaging in murine skin. The utility of this approach was demonstrated by assessment of in vivo caspase-3 activation upon induction of apoptosis. Using this "instant transgenic" model in combination with a corrected coordinate system, we followed the in vivo behavior of individual cells expressing either FRET- or location-based biosensors for several days. Subsequently, the behavior of individual cells expressing these biosensors can be visualized within hours and using conventional microscopy equipment. Resultsīy DNA tattooing we transfect keratinocytes of living mice with DNA encoding fluorescent biosensors. Here, we report a rapid and undemanding intravital imaging method using generally available equipment. However, the generation of transgenic mice is a lengthy process and intravital imaging requires specialized knowledge and equipment. Nor training data acquired for a specific application.Advances in fluorescence microscopy and mouse transgenesis have made it possible to image molecular events in living animals. Reconstruction, the Tattoo approach neither requires complex external hardware Results: An initial feasibility study conducted with experimental phantomĭata and in vivo forearm data indicates that the Tattoo approach is well-suitedįor 3D reconstruction of PAT data with high accuracy and precision.Ĭonclusion: In contrast to previous approaches to 3D ultrasound (US) or PAT This allows the transformation of a sequence of acquired PA images into oneĬommon global coordinate system and thus the consistent 3D reconstruction of
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Recovery of the probe pose relative to the coordinate system of the pattern. This pattern is designed in a way that a tomographic image of it enables the Optical pattern placed on the region of interest prior to image acquisition.
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PAT data (Tattoo tomography) that does not require an external tracking systemĪnd can smoothly be integrated into clinical workflows. Methods: In this paper, we present a novel approach to 3D reconstruction of Promising modality for the diagnosis, treatment and follow-up of variousĭiseases, a current drawback is the limited field-of-view (FoV) provided by the The vessel topology and tissue oxygenation.
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Spatially resolve both morphological and functional tissue properties, such as
Tattoo imaging pdf#
Müller-Stich, Lena Maier-Hein Download PDF Abstract: Purpose: Photoacoustic tomography (PAT) is a novel imaging technique that can Authors: Niklas Holzwarth, Melanie Schellenberg, Janek Gröhl, Kris Dreher, Jan-Hinrich Nölke, Alexander Seitel, Minu D.